Advanced Cryopreservation System for Biological Materials

Publication ID: 24-11856947_0001_PTD
Published: October 26, 2025
Category:Direct Improvements & Enhancements

Legal Citation

pr1or.art Inc., “Advanced Cryopreservation System for Biological Materials,” Published Technical Disclosure No. 24-11856947_0001_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856947_0001_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,947.

Background and Problem Solved

The original patent disclosed a system for automated permeation of biological materials, but it lacked critical features to ensure optimal cryopreservation. The manual procedure of permeating cells into vitrification solutions is time-consuming, labor-intensive, and prone to human error. The new invention addresses these limitations by introducing a temperature control module, real-time monitoring of cryoprotectant agent concentrations, and adaptive flow rate adjustment to minimize exposure time and oxidative stress.

Novelty and Inventive Step

The new claims introduce novel features that significantly improve the original patent's functionality. The temperature control module, real-time monitoring, and adaptive flow rate adjustment are non-obvious enhancements that address the inherent limitations of the original invention. These features provide a more efficient, safe, and reliable cryopreservation process.

Alternative Embodiments and Variations

Alternative embodiments of the invention could include the use of different temperature control methods, such as thermoelectric cooling or heating, or the integration of additional sensors to monitor other parameters, such as pH or oxygen levels. The system could also be adapted for use with different types of biological materials or vitrification solutions.

Potential Commercial Applications and Market

The enhanced system has significant commercial potential in the fields of regenerative medicine, biotechnology, and pharmaceuticals, where cryopreservation of cells and biological materials is a critical step in the development of new therapies and treatments. The market for cryopreservation solutions is growing rapidly, and this invention is well-positioned to capitalize on this trend.

CPC Classifications

SectionClassGroup
A A01 A01N1/0268
A A01 A01N1/0221
A A01 A01N1/0242
B B01 B01L3/502

Field of Art

Biological material preservation, specifically cell cryopreservation techniques involving vitrification and specialized laboratory devices for cell handling and temperature-controlled processing

Person of Ordinary Skill (PHOSITA) Profile

A biomedical engineer or laboratory technician with expertise in cell biology, cryogenic preservation techniques, microfluidic device design, and experience with advanced cell handling protocols

Obviousness Rationale

A person skilled in the art would recognize that the proposed variations represent predictable engineering improvements to the existing automated permeation system, utilizing known techniques in temperature control, sensor integration, and adaptive flow management that are standard design considerations in biological preservation technologies.

Obvious Combinations & Variations

Source Patent Element
Device with reservoirs for biological material permeation
PTD Variation
Adding a temperature control module to regulate solution and material temperature during permeation
Obviousness Reasoning
Temperature management is a known critical parameter in cell preservation, and integrating active temperature control would be an obvious optimization for a PHOSITA seeking to improve vitrification outcomes
Source Patent Element
Manual aspiration and injection of cells into vitrification solutions
PTD Variation
Implementing automated flow rate adjustment based on biological material characteristics
Obviousness Reasoning
Adaptive flow control represents a predictable engineering solution to variability in cell permeation, using standard microfluidic control techniques known in the field
Source Patent Element
Basic device for cell permeation
PTD Variation
Incorporating real-time concentration sensors with alarm systems for cryoprotectant agent monitoring
Obviousness Reasoning
Sensor integration for critical parameter monitoring is a standard design approach in precision biological processing, representing an obvious enhancement to improve process reliability
Source Patent Element
Open system for cell vitrification
PTD Variation
Implementing a controlled atmosphere with reduced oxygen levels to minimize oxidative stress
Obviousness Reasoning
Controlling environmental parameters to reduce cellular damage is a well-understood technique in cell preservation, representing a straightforward and predictable optimization
Source Patent Element
Manual solution preparation for vitrification
PTD Variation
Pre-cooling vitrification solutions to specific temperature ranges between -20°C and -80°C
Obviousness Reasoning
Temperature pre-conditioning of preservation solutions is a known technique for improving cellular survival, representing an obvious method of enhancing the existing preservation protocol
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11856947 and the disclosed technical variations, a person having ordinary skill in the art would find the proposed system and method enhancements to be obvious modifications that do not represent a non-obvious inventive step, thereby rendering potential claims to such variations unpatentable under 35 U.S.C. § 103.

Original Patent Information

Patent NumberUS 11,856,947
TitleSystem for automated permeation of a biological material and method of using same
Assignee(s)Cook Medical Technologies LLC