Published Technical Disclosure Archive

Automated Cryopreservation: Next-Gen Permeation System

Publication ID: 24-11856947_0005_PTD
Published: October 26, 2025
Category:Future Evolutions & Paradigm Shifts

Legal Citation

pr1or.art Inc., “Automated Cryopreservation: Next-Gen Permeation System,” Published Technical Disclosure No. 24-11856947_0005_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856947_0005_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,947.

Background and Problem Solved

The current manual procedure for permeating biological materials with cryoprotectant agents is time-consuming, skill-dependent, and prone to errors. The original patent addressed this issue with a semi-automated device, but its limitations in scalability, customization, and real-time monitoring hinder its widespread adoption. The new invention overcomes these limitations by introducing advanced technologies that enable real-time monitoring, adaptive protocols, and increased automation.

Novelty and Inventive Step

The new claims introduce a paradigm shift in automated permeation technology by integrating AI-driven optimization, real-time monitoring, and modular design. These innovations enable unprecedented levels of automation, customization, and efficiency, making the original patent's semi-automated device obsolete.

Alternative Embodiments and Variations

Alternative embodiments may include the use of different microfluidic materials, alternative machine learning algorithms, or varying swarm robotics configurations. Variations may also include the integration of additional sensors or actuators to further enhance the system's performance and adaptability.

Potential Commercial Applications and Market

The next-generation automated permeation system has vast commercial potential in the fields of cryopreservation, biotechnology, and pharmaceuticals. It can be marketed as a turnkey solution for research institutions, biotech companies, and pharmaceutical manufacturers, enabling them to streamline their cryopreservation processes, reduce costs, and improve product quality.

CPC Classifications

SectionClassGroup
A A01 A01N1/0268
A A01 A01N1/0221
A A01 A01N1/0242
B B01 B01L3/502

Field of Art

Biological material preservation, microfluidic systems, and cryopreservation technologies involving cell permeation and vitrification techniques. Requires expertise in biotechnology, microfluidic engineering, and advanced cell preservation methodologies

Person of Ordinary Skill (PHOSITA) Profile

A bioengineering professional with advanced degree (MS/PhD), expertise in microfluidic design, cell biology, and automated laboratory systems. Familiar with cryopreservation protocols, machine learning applications in biotechnology, and robotic automation techniques

Obviousness Rationale

A PHOSITA would recognize that the PTD's variations represent predictable technological extensions of the source patent's automated permeation system. The core innovations involve integrating known technologies like machine learning, sensor feedback, and modular design to enhance the existing cell permeation methodology. These improvements represent incremental advancements that would be obvious to a skilled practitioner seeking to optimize biological material preservation techniques.

Obvious Combinations & Variations

Source Patent Element
Device with reservoirs for biological material permeation
PTD Variation
Microfluidic platform with integrated sensors for real-time monitoring of cryoprotectant agent concentrations
Obviousness Reasoning
Adding sensors to monitor concentration is a predictable enhancement using known microfluidic technologies. A PHOSITA would recognize the benefit of real-time monitoring to improve permeation precision
Source Patent Element
Manual aspiration and injection of cells into vitrification solutions
PTD Variation
Swarm robotics system to automate permeation process with adaptive programming
Obviousness Reasoning
Automating manual processes using robotic systems is a standard engineering approach. The use of adaptive programming represents a known technique for improving system flexibility
Source Patent Element
Device made of clear or translucent plastic with visualization capabilities
PTD Variation
3D-printed modular design with interchangeable reservoirs and sensors
Obviousness Reasoning
Modular design and advanced manufacturing techniques are predictable evolutionary steps in biotechnology device development. A PHOSITA would recognize 3D printing as an obvious method to create customizable laboratory equipment
Source Patent Element
Device with reservoirs configured for suction or positive pressure
PTD Variation
Closed-loop feedback system utilizing real-time sensor data and machine learning algorithms
Obviousness Reasoning
Implementing feedback control systems is a standard engineering approach. Machine learning represents an obvious extension of existing control system technologies to optimize biological processes
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11856947 and the disclosed technological variations, a person of ordinary skill in the art would find the claimed innovations obvious. The proposed system represents predictable combinations of known techniques in microfluidic design, robotic automation, and adaptive control systems, thereby rendering subsequent patent claims obvious and unpatentable under 35 U.S.C. Section 103.

Original Patent Information

Patent NumberUS 11,856,947
TitleSystem for automated permeation of a biological material and method of using same
Assignee(s)Cook Medical Technologies LLC