Published Technical Disclosure Archive

Advanced Automated Cell Cryopreservation System

Publication ID: 24-11856947_0006_PTD
Published: October 26, 2025
Category:Direct Improvements & Enhancements

Legal Citation

pr1or.art Inc., “Advanced Automated Cell Cryopreservation System,” Published Technical Disclosure No. 24-11856947_0006_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856947_0006_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,947.

Background and Problem Solved

The current manual procedure for cryopreserving cells through vitrification is labor-intensive, prone to human error, and limited by the inherent design of existing devices. The original patent, 'System for automated permeation of a biological material and method of using same,' addresses this issue but has limitations in terms of temperature control, automation, and component replacement. The new invention overcomes these limitations by introducing advanced features that make the permeation process faster, safer, and more efficient.

Novelty and Inventive Step

The new invention introduces a novel combination of advanced features, including temperature control, robotic assistance, sensor-based concentration adjustment, built-in pumping, and modular design. These features overcome the limitations of the original patent and provide a significant improvement in the automated permeation of biological materials.

Alternative Embodiments and Variations

Alternative embodiments of the invention could include varying the type of temperature control element, using different robotic arm configurations, incorporating additional sensors for monitoring other parameters, or modifying the pump design for specific applications. The modular design allows for easy adaptation to different cryopreservation protocols and biological materials.

Potential Commercial Applications and Market

The enhanced system has significant commercial potential in the fields of regenerative medicine, biotechnology, and pharmaceuticals, where cryopreservation of biological materials is crucial. The improved efficiency, safety, and reliability of the system make it an attractive solution for industries seeking to optimize their cryopreservation processes.

CPC Classifications

SectionClassGroup
A A01 A01N1/0268
A A01 A01N1/0221
A A01 A01N1/0242
B B01 B01L3/502

Field of Art

Biological material preservation and cryogenic processing, specifically involving cell vitrification techniques, requiring expertise in biotechnology, laboratory techniques, and precision fluid handling systems

Person of Ordinary Skill (PHOSITA) Profile

A biomedical engineer or laboratory technician with advanced training in cell biology, experience with cryopreservation protocols, understanding of microfluidic device design, and knowledge of automated laboratory instrumentation

Obviousness Rationale

A person having ordinary skill would recognize that the source patent's automated permeation device provides a foundational design that can be incrementally enhanced through predictable technological improvements. The PTD's variations represent straightforward extensions of the original patent's core concepts, utilizing standard engineering approaches to address known limitations in biological material processing. These modifications would be considered routine optimization by a skilled practitioner familiar with cryopreservation technology.

Obvious Combinations & Variations

Source Patent Element
Device with lower and upper portions configured for biological material processing
PTD Variation
Adding temperature control element to maintain optimal cryopreservation conditions
Obviousness Reasoning
Temperature regulation is a known critical parameter in cell preservation, and integrating a temperature control mechanism would be an obvious design improvement for a skilled engineer seeking to enhance preservation outcomes
Source Patent Element
Manual aspiration and injection of biological materials
PTD Variation
Implementing robotic arm for automated aspiration and injection processes
Obviousness Reasoning
Automation of repetitive laboratory procedures is a predictable technological progression, and replacing manual handling with robotic systems represents a standard approach to improving precision and reducing human error
Source Patent Element
Device for transferring biological materials between reservoirs
PTD Variation
Incorporating sensor-based concentration detection and automatic adjustment of cryoprotectant agents
Obviousness Reasoning
Implementing real-time monitoring and adaptive concentration control is a logical extension of existing process control techniques, representing a predictable solution to improving permeation consistency
Source Patent Element
Device with releasably coupled upper and lower portions
PTD Variation
Creating a modular design allowing easy component replacement and increased system adaptability
Obviousness Reasoning
Modular design is a standard engineering approach to improving equipment flexibility and reducing maintenance downtime, representing a straightforward optimization for laboratory instrumentation
Source Patent Element
Manual fluid transfer between reservoirs
PTD Variation
Integrating built-in pump with controlled flow rate for precise material permeation
Obviousness Reasoning
Implementing integrated pumping mechanisms with precise flow control is a predictable technological improvement for ensuring consistent and controlled biological material processing
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11856947 and the disclosed technical variations, a person having ordinary skill in the art would find the claimed innovations obvious and lacking non-obvious inventive merit. The incremental technological enhancements represent routine optimization within the established technological framework of biological material processing devices, thereby rendering potential derivative claims obvious and unpatentable under 35 U.S.C. ยง 103.

Original Patent Information

Patent NumberUS 11,856,947
TitleSystem for automated permeation of a biological material and method of using same
Assignee(s)Cook Medical Technologies LLC