Advanced Automated Cell Cryopreservation Technology

Publication ID: 24-11856947_0010_PTD
Published: October 26, 2025
Category:Future Evolutions & Paradigm Shifts

Legal Citation

pr1or.art Inc., “Advanced Automated Cell Cryopreservation Technology,” Published Technical Disclosure No. 24-11856947_0010_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856947_0010_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,947.

Background and Problem Solved

Current methods for cryopreserving cells through vitrification rely on manual procedures, which are time-consuming, labor-intensive, and prone to human error. The original patent addressed this issue with a device for automated permeation, but its limitations in terms of precision, efficiency, and adaptability to different cell types and cryoprotectant concentrations remain. This new invention overcomes these limitations by integrating advanced technologies to create a more efficient, accurate, and customizable permeation process.

Novelty and Inventive Step

The new claims introduce a paradigm shift in cryopreservation technology by integrating robotics, artificial intelligence, and microfluidics to create a highly efficient, adaptable, and precise permeation process. The use of machine learning algorithms to optimize permeation protocols, electroporation to enhance cryoprotectant uptake, and interchangeable cartridges for customized protocols are all novel and non-obvious advancements over the original patent.

Alternative Embodiments and Variations

Alternative embodiments of the invention could include the use of other types of automation, such as pneumatic or hydraulic systems, or the integration of additional sensors and monitoring systems to further optimize the permeation process. Variations of the invention could also include the use of different types of cryoprotectant agents, or the application of the technology to other types of biological materials.

Potential Commercial Applications and Market

The advanced system for automated permeation of biological materials has significant commercial potential in the fields of regenerative medicine, biobanking, and cryopreservation. The technology could be used to improve the efficiency and accuracy of cryopreservation processes, leading to increased adoption and market growth. Potential target industries include pharmaceutical companies, research institutions, and biotechnology companies.

CPC Classifications

SectionClassGroup
A A01 A01N1/0268
A A01 A01N1/0221
A A01 A01N1/0242
B B01 B01L3/502

Field of Art

Biological material preservation and cryogenic processing, specifically cell vitrification techniques involving microfluidic systems, automation, and cryoprotectant permeation protocols

Person of Ordinary Skill (PHOSITA) Profile

A biomedical engineer or biotechnologist with expertise in cell preservation techniques, microfluidic device design, robotic automation, and machine learning applications in biological processing, holding advanced degrees in bioengineering or related fields

Obviousness Rationale

A PHOSITA would recognize that the disclosed variations represent predictable technological extensions of the source patent's core permeation system, utilizing standard engineering approaches like robotics, machine learning, and microfluidic optimization to address known limitations in manual cell preservation processes. The proposed modifications represent incremental improvements using well-established techniques in biotechnology and automation. The variations demonstrate systematic problem-solving approaches that would be obvious to a skilled practitioner seeking to enhance the efficiency and precision of cell vitrification methods.

Obvious Combinations & Variations

Source Patent Element
Manual aspiration and injection of cells into vitrification solutions
PTD Variation
Robotic arm configured to automatically aspirate and inject cells with machine learning optimization
Obviousness Reasoning
Automation of manual processes is a predictable solution in biotechnology, with robotic systems being a known technique for reducing human error and increasing precision in laboratory procedures
Source Patent Element
Static device for supporting biological materials during permeation
PTD Variation
Modular permeation device with interchangeable cartridges and customizable protocols
Obviousness Reasoning
Modular design is a standard engineering approach for increasing system flexibility, and a PHOSITA would recognize the benefits of adaptable cryopreservation platforms
Source Patent Element
Basic permeation device with reservoirs for biological materials
PTD Variation
Microfluidic chamber utilizing electroporation with real-time feedback monitoring
Obviousness Reasoning
Enhancement of permeation techniques through advanced physical methods like electroporation is a predictable evolution in cell preservation technology, representing a finite set of known optimization strategies
Source Patent Element
Manual concentration gradient creation for cryoprotectant solutions
PTD Variation
Machine learning algorithm predicting optimal cryoprotectant concentrations based on cell type and parameters
Obviousness Reasoning
Application of machine learning to optimize biological protocols is a standard approach in biotechnology, representing an obvious extension of existing process optimization techniques
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11856947 and the disclosed variations, a person of ordinary skill in the art would find the proposed cryopreservation system modifications to be obvious extensions of existing technology. The systematic integration of robotics, machine learning, and microfluidic techniques represents predictable technological improvements that would be readily conceived by a skilled practitioner seeking to enhance cell vitrification processes.

Original Patent Information

Patent NumberUS 11,856,947
TitleSystem for automated permeation of a biological material and method of using same
Assignee(s)Cook Medical Technologies LLC