Advanced Microbial Oil Purification Technology

Publication ID: 24-11856952_0001_PTD
Published: October 27, 2025
Category:Direct Improvements & Enhancements

Legal Citation

pr1or.art Inc., “Advanced Microbial Oil Purification Technology,” Published Technical Disclosure No. 24-11856952_0001_PTD, Published October 27, 2025, available at https://archive.pr1or.art/24-11856952_0001_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,952.

Background and Problem Solved

The original patent disclosed a microbial oil composition with high contents of arachidonic acid (ARA) and Mead acid. However, the production and purification process of the microbial oil had limitations, such as low yields and inefficient purification methods. The present invention addresses these limitations by introducing a novel rectification column with optimized temperature and pressure conditions, enabling real-time monitoring and control of the purification process.

Novelty and Inventive Step

The novelty of the present invention lies in the combination of the rectification column with optimized temperature and pressure conditions, and the real-time monitoring and control of the purification process. This invention provides a significant improvement over the original patent by enhancing the yield and quality of the microbial oil, while improving the efficiency and safety of the process.

Alternative Embodiments and Variations

Alternative embodiments of the invention may include different types of purification columns, such as packed columns or tray columns, and varying temperature and pressure conditions. Additionally, the invention may be adapted for use with different types of microorganisms or starting oils.

Potential Commercial Applications and Market

The invention has significant commercial potential in the food, pharmaceutical, and cosmetic industries, where high-quality microbial oil is in high demand. The improved yield and quality of the microbial oil, combined with the enhanced efficiency and safety of the process, make this invention an attractive solution for companies seeking to produce high-value products.

CPC Classifications

SectionClassGroup
A A01 A01N37/06
A A23 A23D9/013
A A23 A23D9/02
A A23 A23K20/158
A A23 A23L33/115
A A61 A61K8/361
A A61 A61K8/9706
A A61 A61K8/9728
A A61 A61K8/99
A A61 A61K31/202
A A61 A61K36/06
A A61 A61Q19/00
C C11 C11B1/025
C C11 C11B3/10
C C11 C11B3/12
C C11 C11C1/005
C C11 C11C3/10
C C12 C12N1/28
C C12 C12P7/6427
C C12 C12P7/6436
C C12 C12P7/6463

Field of Art

Biotechnology and food science, specifically microbial oil production and fatty acid extraction, involving advanced biochemical engineering techniques for cultivating and processing microorganisms to produce specialized lipid compositions

Person of Ordinary Skill (PHOSITA) Profile

A skilled practitioner with expertise in microbial fermentation, lipid chemistry, and industrial separation techniques, holding at least a master's degree in biochemical engineering or related field, with working knowledge of advanced extraction and purification methodologies

Obviousness Rationale

A person of ordinary skill would recognize that the PTD's disclosed rectification and purification variations represent predictable engineering optimizations of the source patent's microbial oil production process. The temperature and pressure modifications represent standard process engineering approaches to enhancing fatty acid extraction and concentration. The real-time monitoring and control techniques are consistent with established industrial process improvement strategies in biotechnological manufacturing.

Obvious Combinations & Variations

Source Patent Element
Microbial oil production method involving fatty acid extraction
PTD Variation
Introducing specific rectification column temperature range of 160°C to 230°C and pressure range of 0.1 Pa to 30 Pa
Obviousness Reasoning
Routine process parameter optimization using known engineering principles of temperature and pressure manipulation to improve extraction efficiency
Source Patent Element
Fatty acid concentration techniques
PTD Variation
Real-time monitoring and control of purification process
Obviousness Reasoning
Standard industrial control methodology representing predictable application of process monitoring technologies to improve manufacturing consistency
Source Patent Element
Microbial oil containing polyunsaturated fatty acids
PTD Variation
Adding antioxidants to improve oil stability
Obviousness Reasoning
Well-known preservation technique in lipid chemistry for preventing oxidative degradation of unsaturated fatty acid compositions
Source Patent Element
Microbial cultivation for oil production
PTD Variation
Optimizing microorganism cultivation conditions to increase polyunsaturated fatty acid production
Obviousness Reasoning
Standard bioengineering approach of iterative process improvement through systematic cultivation parameter modification
Source Patent Element
Fatty acid extraction using separation techniques
PTD Variation
Exploring alternative purification columns like packed or tray columns
Obviousness Reasoning
Predictable design variation using known industrial separation equipment with expected equivalent performance characteristics
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11856952, the present publication demonstrates that the claimed microbial oil production variations would have been obvious to a person having ordinary skill in the art at the time of invention, as the technical modifications represent predictable engineering optimizations using known biotechnological processing techniques and standard industrial process improvement methodologies.

Original Patent Information

Patent NumberUS 11,856,952
TitleMicrobial oil, production method for microbial oil, concentrated microbial oil, and production method for concentrated microbial oil
Assignee(s)NIPPON SUISAN KAISHA, LTD.