Published Technical Disclosure Archive

Recombinant Bacillus: Multi-Industry Biotech Innovation

Publication ID: 24-11856956_0007_PTD
Published: October 26, 2025
Category:New Applications & Use Cases

Legal Citation

pr1or.art Inc., “Recombinant Bacillus: Multi-Industry Biotech Innovation,” Published Technical Disclosure No. 24-11856956_0007_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856956_0007_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,956.

Background and Problem Solved

The original patent focused on compositions comprising recombinant Bacillus cells and an insecticide for agricultural purposes. However, the full potential of these cells has not been explored in other industries. This invention addresses the limitations of the original patent by applying the core technology to new, unrelated fields, thereby expanding its commercial reach and social impact.

Novelty and Inventive Step

The new claims introduce novel applications and use cases that were not considered in the original patent, thereby providing a non-obvious expansion of the technology's scope. The inventive step lies in recognizing the potential of recombinant Bacillus cells in unrelated fields and adapting the core technology to address specific challenges in each industry.

Alternative Embodiments and Variations

Alternative embodiments may include variations in the type of insecticide used, the specific exosporium targeting sequence, or the fusion protein's design. The invention could also be implemented using different Bacillus species or strains, or by incorporating additional components to enhance its efficacy.

Potential Commercial Applications and Market

The invention has significant commercial potential in controlled-environment agriculture, human health, bioremediation, pest control, and pharmaceuticals, with potential markets including vertically-stacked plant growth facilities, gut health supplements, bioremediation services, precision agriculture, and pharmaceutical companies.

CPC Classifications

SectionClassGroup
A A01 A01N63/23
A A01 A01N37/40
A A01 A01N37/52
A A01 A01N41/10
A A01 A01N43/12
A A01 A01N43/24
A A01 A01N43/40
A A01 A01N43/56
A A01 A01N43/653
A A01 A01N43/713
A A01 A01N47/06
A A01 A01N47/22
A A01 A01N47/24
A A01 A01N47/34
A A01 A01N57/08
A A01 A01N57/14
A A01 A01N63/50
A A01 A01N51/00
A A01 A01N55/00

Field of Art

Agricultural biotechnology, microbial engineering, and pest control with a focus on recombinant Bacillus cell technologies and biological pest management strategies

Person of Ordinary Skill (PHOSITA) Profile

A skilled practitioner with advanced degrees in microbiology, genetic engineering, or agricultural sciences, possessing expertise in recombinant DNA techniques, bacterial protein expression, and biological control mechanisms

Obviousness Rationale

A person of ordinary skill would recognize that the core technology of recombinant Bacillus cells with fusion proteins can be readily adapted to multiple application domains by systematically substituting target environments or biological objectives. The source patent establishes a fundamental platform technology that enables predictable variations across different technical contexts. The disclosed variations represent straightforward extrapolations of the original invention's core principles of targeted protein expression and insecticidal functionality.

Obvious Combinations & Variations

Source Patent Element
Recombinant Bacillus cells expressing fusion proteins with endoglucanase and exosporium targeting sequences
PTD Variation
Applying the same cellular engineering approach to controlled-environment agriculture and vertical farming systems
Obviousness Reasoning
Predictable extension of existing biotechnology platform using known genetic manipulation techniques to address agricultural productivity challenges
Source Patent Element
Insecticide-containing Bacillus cell compositions
PTD Variation
Repurposing the cellular system for bioremediation and soil pollutant degradation
Obviousness Reasoning
Known microbial engineering strategy of adapting bacterial metabolic capabilities to environmental remediation applications
Source Patent Element
Fusion protein design with targeting sequences
PTD Variation
Implementing real-time pest monitoring systems using sensor-integrated bacterial detection mechanisms
Obviousness Reasoning
Obvious combination of existing biotechnology with Internet of Things (IoT) sensing technologies using standard engineering design approaches
Source Patent Element
Recombinant Bacillus cells capable of protein expression
PTD Variation
Utilizing the cellular system for pharmaceutical bioactive compound production
Obviousness Reasoning
Well-established biotechnological technique of leveraging bacterial expression systems for industrial and pharmaceutical manufacturing
Source Patent Element
Fusion protein comprising endoglucanase and targeting sequences
PTD Variation
Exploring potential human microbiome modulation applications
Obviousness Reasoning
Predictable exploration of bacterial protein technologies for potential health interventions using standard bioengineering methodologies
35 U.S.C. § 103 Summary: Pursuant to 35 U.S.C. ยง 103, the variations disclosed herein would have been obvious to a person having ordinary skill in the art at the time of the invention, as they represent predictable extensions of the foundational technology disclosed in US Patent 11856956. The published technical disclosure demonstrates that the claimed innovations emerge from routine application of known recombinant bacterial engineering principles, thereby establishing anticipatory prior art against subsequent patent claims in this technological domain.

Original Patent Information

Patent NumberUS 11,856,956
TitleCompositions comprising recombinant Bacillus cells and an insecticide
Assignee(s)BASF SE