Advanced Recombinant Bacillus Pest Control Solutions

Publication ID: 24-11856956_0009_PTD
Published: October 26, 2025
Category:Specialized Variations & Niche Solutions

Legal Citation

pr1or.art Inc., “Advanced Recombinant Bacillus Pest Control Solutions,” Published Technical Disclosure No. 24-11856956_0009_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856956_0009_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,956.

Background and Problem Solved

The original patent disclosed compositions comprising recombinant Bacillus cells and an insecticide, but these compositions had limited adaptability to specific, challenging operational environments. This invention addresses the need for tailored solutions in extreme weather conditions, high-security areas, and disaster relief scenarios, where conventional pest control and crop protection methods may be inadequate.

Novelty and Inventive Step

The new claims introduce novel, non-obvious adaptations of the original composition, specifically tailored to address the unique challenges of extreme weather conditions, high-security areas, and disaster relief scenarios. These adaptations include the selection of specific insecticides, the optimization of the recombinant Bacillus cells for extreme environments, and the integration of these components into systems and methods for specialized applications.

Alternative Embodiments and Variations

Alternative embodiments of the invention could include the use of different insecticides, varying the sequence identity of the endoglucanase, or incorporating additional components to enhance the composition's effectiveness in specific environments. Variations of the system and method could include adjustments to the application protocols, the type of crops protected, or the specific disaster relief scenarios addressed.

Potential Commercial Applications and Market

The invention has significant commercial potential in the agricultural, biotechnology, and disaster relief industries, particularly in regions prone to extreme weather conditions or high-security areas. The specialized compositions and systems could be marketed as premium products, offering enhanced pest control and crop protection in these niche markets.

CPC Classifications

SectionClassGroup
A A01 A01N63/23
A A01 A01N37/40
A A01 A01N37/52
A A01 A01N41/10
A A01 A01N43/12
A A01 A01N43/24
A A01 A01N43/40
A A01 A01N43/56
A A01 A01N43/653
A A01 A01N43/713
A A01 A01N47/06
A A01 A01N47/22
A A01 A01N47/24
A A01 A01N47/34
A A01 A01N57/08
A A01 A01N57/14
A A01 A01N63/50
A A01 A01N51/00
A A01 A01N55/00

Field of Art

Agricultural biotechnology, specifically microbial pest control compositions involving recombinant Bacillus cells and insecticide formulations, with expertise in genetic engineering, protein expression, and agricultural chemical applications

Person of Ordinary Skill (PHOSITA) Profile

A researcher with advanced degrees in microbiology or agricultural biotechnology, familiar with genetic manipulation of Bacillus species, protein engineering techniques, and insecticide formulation strategies, with practical experience in developing microbial pest control solutions

Obviousness Rationale

A person of ordinary skill would recognize that the PTD's variations represent predictable extensions of the source patent's core technology by systematically exploring alternative insecticide combinations, environmental adaptations, and application contexts while maintaining the fundamental recombinant Bacillus cell and fusion protein structure disclosed in the original patent.

Obvious Combinations & Variations

Source Patent Element
Recombinant Bacillus cells expressing a fusion protein with endoglucanase and exosporium targeting sequence
PTD Variation
Adapting the composition for specific environmental conditions like extreme temperatures or high soil salinity
Obviousness Reasoning
A PHOSITA would understand that microbial compositions can be readily optimized for different environmental conditions through routine experimentation, representing a predictable variation of known biotechnological techniques
Source Patent Element
Insecticide selection from a defined group including clothianidin, cypermethrin, fipronil, and imidacloprid
PTD Variation
Selecting specific subsets of insecticides for targeted application scenarios like disaster relief or high-security crop protection
Obviousness Reasoning
Choosing specific insecticides from a known set for particular use cases represents an obvious design choice based on known properties and performance characteristics
Source Patent Element
Fusion protein with endoglucanase having at least 90% sequence identity to SEQ ID NO: 107
PTD Variation
Maintaining the core protein sequence while exploring alternative application contexts and environmental adaptations
Obviousness Reasoning
Preserving the fundamental protein engineering approach while expanding potential use cases is a standard approach in biotechnological innovation, representing an obvious technical progression
Source Patent Element
Bacillus cereus family cell-based composition
PTD Variation
Specialized systems for disaster relief, high-security areas, and extreme environmental conditions
Obviousness Reasoning
Adapting known microbial compositions to specific application scenarios through minor modifications represents a predictable engineering approach within the technical field
35 U.S.C. § 103 Summary: Pursuant to 35 U.S.C. ยง 103, the variations disclosed in this publication would be considered obvious to a person having ordinary skill in the art at the time of invention, as they represent predictable extensions of the foundational technology disclosed in US Patent 11856956, involving routine optimization of recombinant Bacillus cell compositions through standard biotechnological techniques and design choices.

Original Patent Information

Patent NumberUS 11,856,956
TitleCompositions comprising recombinant Bacillus cells and an insecticide
Assignee(s)BASF SE