Published Technical Disclosure Archive

Bacillus Subtilis: Innovative Environmental Remediation Tech

Publication ID: 24-11856970_0002_PTD
Published: October 26, 2025
Category:New Applications & Use Cases

Legal Citation

pr1or.art Inc., “Bacillus Subtilis: Innovative Environmental Remediation Tech,” Published Technical Disclosure No. 24-11856970_0002_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856970_0002_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,970.

Background and Problem Solved

The original patent disclosed the use of Bacillus subtilis strain DSM 32685 in animal feed to inhibit bacterial colonization and improve animal performance. However, the environmental and public health implications of this strain remained unexplored. This invention addresses the limitations of the original patent by applying the core technology to new industries and problems, providing innovative solutions for environmental remediation and public health.

Novelty and Inventive Step

The new claims demonstrate novelty and inventive step by expanding the use of Bacillus subtilis strain DSM 32685 beyond animal feed, providing innovative solutions for environmental remediation and public health. The specific combinations of the strain with different applications and industries are non-obvious and represent a significant departure from the original patent.

Alternative Embodiments and Variations

Alternative embodiments of the invention could include using different strains of Bacillus subtilis, combining the strain with other microorganisms or chemicals, or applying the strain to different environmental or public health contexts. Variations of the invention could also include using the strain in agricultural settings, such as reducing soil pollution or improving crop yields.

Potential Commercial Applications and Market

The invention has significant commercial potential in various industries, including environmental remediation, public health, agriculture, and industrial wastewater treatment. The market for these applications is substantial, with growing demand for sustainable and effective solutions to environmental and public health challenges.

CPC Classifications

SectionClassGroup
A A23 A23K50/40
A A01 A01N63/22
A A23 A23K10/18
C C12 C12N1/205
C C12 C12R2001/125

Field of Art

Microbiology, biotechnology, and environmental remediation with a focus on bacterial strain applications in agriculture, environmental management, and public health

Person of Ordinary Skill (PHOSITA) Profile

A microbiologist or biotechnologist with expertise in bacterial strain characterization, with knowledge of Bacillus subtilis applications, microbial interactions, and environmental biotechnology techniques

Obviousness Rationale

A PHOSITA would recognize that the Bacillus subtilis DSM 32685 strain's demonstrated antimicrobial and environmental interaction capabilities inherently suggest broader applications beyond animal feed. The strain's proven ability to inhibit pathogen growth and interact with environmental systems provides a clear technical foundation for exploring alternative uses in water treatment, soil remediation, and infection control.

Obvious Combinations & Variations

Source Patent Element
Bacillus subtilis strain DSM 32685 with demonstrated pathogen inhibition capabilities
PTD Variation
Application to water pollution reduction and aquatic pathogen control
Obviousness Reasoning
The strain's inherent antimicrobial properties make its extension to water treatment a predictable and obvious technical solution using known microbiological techniques
Source Patent Element
Bacterial strain capable of inhibiting multiple bacterial species in animal feed
PTD Variation
Use in reducing hospital-acquired infections by inhibiting nosocomial pathogens
Obviousness Reasoning
The strain's demonstrated broad-spectrum antimicrobial activity naturally suggests application in infection control settings as a known and predictable extension of its core biological characteristics
Source Patent Element
Bacillus subtilis strain with ability to interact with biological systems
PTD Variation
Bioremediation of contaminated soil by breaking down pollutants
Obviousness Reasoning
A PHOSITA would recognize the strain's potential for environmental interaction as an obvious approach to soil remediation using standard biotechnological principles
Source Patent Element
Method of administering bacterial spores in animal feed compositions
PTD Variation
Composition for reducing indoor air pollution using bacterial spores
Obviousness Reasoning
The strain's stability as spores and proven ability to interact with biological systems makes its application to air quality management a predictable design choice
35 U.S.C. § 103 Summary: Based on US Patent 11856970 disclosing Bacillus subtilis DSM 32685, the present publication demonstrates that a person of ordinary skill in the art would find the expanded applications of said bacterial strain in environmental and health contexts to be obvious variations of the original invention, thereby establishing prior art that would render similar claimed innovations non-patentable under 35 U.S.C. Section 103.

Original Patent Information

Patent NumberUS 11,856,970
TitleBacillus subtilis for animal feed
Assignee(s)Chr. Hansen A/S