Published Technical Disclosure Archive

Advanced Bacillus Subtilis Feed Innovation

Publication ID: 24-11856970_0010_PTD
Published: October 26, 2025
Category:Future Evolutions & Paradigm Shifts

Legal Citation

pr1or.art Inc., “Advanced Bacillus Subtilis Feed Innovation,” Published Technical Disclosure No. 24-11856970_0010_PTD, Published October 26, 2025, available at https://archive.pr1or.art/24-11856970_0010_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,856,970.

Background and Problem Solved

The original patent disclosed the use of Bacillus subtilis for inhibiting bacterial colonization and improving animal performance. However, it had limitations in terms of antibiotic susceptibility, lack of customization, and limited monitoring capabilities. The present invention addresses these limitations by introducing a modular system that combines gene editing tools, customized Bacillus subtilis strains, and real-time monitoring sensors to provide a more effective and sustainable solution for animal nutrition and welfare.

Novelty and Inventive Step

The present invention introduces a modular system that integrates gene editing tools, customized Bacillus subtilis strains, and real-time monitoring sensors, providing a novel and non-obvious solution for animal nutrition and welfare. The use of gene editing tools to modify the antibiotic susceptibility profile of the Bacillus subtilis strain is a significant departure from the original patent and offers a new level of customization and efficacy.

Alternative Embodiments and Variations

Alternative embodiments of the invention could include the use of different gene editing tools, such as CRISPR-Cas9 or TALEN, or the integration of additional sensors for monitoring other aspects of animal health. Variations of the invention could also include the use of different Bacillus subtilis strains or the development of new compositions for enhancing animal welfare.

Potential Commercial Applications and Market

The present invention has significant commercial potential in the animal feed industry, offering a more effective and sustainable solution for animal nutrition and welfare. The invention could be marketed to animal feed manufacturers, farmers, and veterinarians, and could also be used in human health applications, such as probiotics and personalized nutrition.

CPC Classifications

SectionClassGroup
A A23 A23K50/40
A A01 A01N63/22
A A23 A23K10/18
C C12 C12N1/205
C C12 C12R2001/125

Field of Art

Animal nutrition biotechnology, focusing on probiotic microorganism development for animal feed, with expertise in microbiology, genetic engineering, and microbiome modulation

Person of Ordinary Skill (PHOSITA) Profile

A researcher with advanced degrees in microbiology or biotechnology, familiar with gene editing techniques, probiotic strain development, and animal nutrition science, possessing skills in molecular biology and microbiological strain manipulation

Obviousness Rationale

A person of ordinary skill would recognize that gene editing tools provide a predictable method for modifying Bacillus subtilis strains' characteristics, extending the source patent's foundational probiotic technology. The integration of sensor technologies and personalized nutrition approaches represents a natural progression of existing microbiome management strategies. The disclosed variations demonstrate incremental improvements using known biotechnological techniques that would be apparent to a skilled practitioner in the field.

Obvious Combinations & Variations

Source Patent Element
Bacillus subtilis strain DSM 32685 with antibiotic susceptibility
PTD Variation
Gene editing tools for targeted modification of antibiotic susceptibility profile
Obviousness Reasoning
CRISPR and similar gene editing techniques are well-established for precise genetic modifications, making strain customization a predictable extension of existing biotechnology practices
Source Patent Element
Method of administering Bacillus subtilis for animal performance improvement
PTD Variation
Real-time monitoring sensors and personalized nutrition platform
Obviousness Reasoning
Integrating sensor technologies with probiotic delivery represents a logical advancement in precision animal nutrition, utilizing known data analytics and monitoring techniques
Source Patent Element
Composition including Bacillus subtilis with other bacterial strains
PTD Variation
Modular system for in-situ production of customized probiotic strains
Obviousness Reasoning
Bioreactor technologies and strain development are standard practices in biotechnology, making a customizable production system an obvious technological progression
Source Patent Element
Method of inhibiting bacterial colonization in animal feed
PTD Variation
Machine learning algorithm for predicting optimal feed compositions
Obviousness Reasoning
Computational approaches to microbiome optimization are well-established, representing a predictable application of data science to existing probiotic technologies
Source Patent Element
Bacillus subtilis spore-based delivery method
PTD Variation
Gene editing tools for enhancing spore stability and targeted delivery
Obviousness Reasoning
Genetic modification of bacterial delivery mechanisms is a known technique for improving probiotic efficacy and represents a foreseeable technological improvement
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11856970 and the disclosed technological variations, a person of ordinary skill in the art would find the claimed innovations obvious, as they represent predictable extensions of existing probiotic strain development and animal nutrition technologies, utilizing standard gene editing, sensor integration, and computational techniques known in the biotechnology field.

Original Patent Information

Patent NumberUS 11,856,970
TitleBacillus subtilis for animal feed
Assignee(s)Chr. Hansen A/S