RNA-based solutions for environmental monitoring and remediation

The present inventive concept leverages RNA replicon technology to develop innovative solutions for environmental monitoring, remediation, and sustainability, addressing pressing ecological challenges.

The original patent focused on RNA delivery for immunization. However, the environmental sector faces significant challenges in monitoring and remediating pollution, invasive species, and disease in plants and aquatic ecosystems. The present inventive concept tackles these issues by applying RNA replicon technology to develop novel systems and methods for environmental sustainability.

The inventive concept comprises four primary aspects: (1) a system for monitoring water quality using underwater sensors and RNA replicon-based data analysis; (2) a method for treating contaminated soil via RNA replicon-encoded proteins that degrade pollutants; (3) a device for detecting biomarkers of disease in plants using portable RNA replicon-based sensors; and (4) a system for improving crop yields through RNA replicon-encoded proteins that enhance plant growth. These innovations harness the power of RNA replicons to trigger immune responses in aquatic organisms, plants, and soil microorganisms, enabling effective environmental monitoring and remediation.

The present inventive concept introduces a paradigm shift by applying RNA replicon technology to environmental applications, departing from the original patent's focus on immunization. The new claims demonstrate a non-obvious and innovative use of RNA replicons, leveraging their immunogenic properties to address ecological challenges.

Alternative embodiments may include RNA replicon-based systems for air quality monitoring, waste management, or bioremediation. Variations could involve the use of different RNA replicon architectures, delivery methods, or sensor technologies to adapt to specific environmental contexts.

The present inventive concept has significant commercial potential in the environmental sector, with potential applications in water quality monitoring, soil remediation, plant disease detection, and crop yield enhancement. The target market includes government agencies, environmental consulting firms, agricultural companies, and research institutions.