Enhanced Lactobacillus Paracasei MG4272 Strain for Improved Antimicrobial Activity

The inventive concept discloses novel methods and compositions that enhance the antimicrobial activity of Lactobacillus paracasei MG4272 strain against Gardnerella vaginalis and Candida albicans, addressing the limitations of the original patent.

The original patent disclosed a composition comprising a cell-free supernatant of Lactobacillus paracasei MG4272 strain, which has antimicrobial activity against Gardnerella vaginalis and Candida albicans. However, the original patent has limitations in terms of the strain's stability in acidic environments, autoaggregation ability, and sugar fermentation activity. The new inventive concept addresses these limitations by providing methods and compositions that enhance the antimicrobial activity of the Lactobacillus paracasei MG4272 strain.

The new inventive concept includes a method for enhancing the antimicrobial effect of Lactobacillus paracasei MG4272 strain by administering a combination of the strain and a prebiotic to a subject. Additionally, the concept includes a system for improving the stability of the strain in acidic environments using a buffer solution and a pH regulator. Furthermore, the concept discloses a composition comprising a cell-free supernatant of the genetically modified Lactobacillus paracasei MG4272 strain to produce a higher amount of antimicrobial peptides. The concept also includes a method for increasing the autoaggregation ability of the strain by treating it with a peptide-based adhesion molecule. Finally, the concept includes a kit for detecting the presence of Lactobacillus paracasei MG4272 strain in a biological sample using a primer set specific to the strain and a detection reagent.

The new inventive concept is novel and non-obvious compared to the original patent because it provides specific solutions to the limitations of the original patent, including enhancing the antimicrobial activity, improving stability, increasing autoaggregation ability, and detecting the presence of the strain. The new claims are not obvious from the original patent and provide a significant improvement over the existing art.

Alternative embodiments of the inventive concept could include using different prebiotics, buffer solutions, or pH regulators to enhance the antimicrobial activity and stability of the Lactobacillus paracasei MG4272 strain. Additionally, the concept could be implemented using different genetic modifications to produce antimicrobial peptides or using different peptide-based adhesion molecules to increase autoaggregation ability.

The inventive concept has significant commercial potential in the fields of pharmaceuticals, nutrition, and biotechnology. The enhanced antimicrobial activity of the Lactobacillus paracasei MG4272 strain could be used to develop novel therapeutic products for the treatment of vaginal infections and other diseases. The concept could also be used to develop novel probiotic products with improved stability and autoaggregation ability.