RNA-based solutions for industrial, agricultural, and biomedical applications

The inventive concept applies the core technology of self-replicating cell selective gene delivery compositions to novel industries, including industrial wastewater treatment, crop yield enhancement, skin disorder treatment, infectious disease detection, and biofuel production.

The original patent focused on therapeutic strategies for human diseases. However, the limitations of the original patent in terms of applicability to other fields have been addressed by the new claims, which explore the potential of RNA-based solutions in various industrial, agricultural, and biomedical applications. The new inventive concept tackles specific problems in these fields, such as pollutant degradation, environmental stress, skin cell differentiation, pathogen detection, and biofuel production.

The new inventive concept comprises polyribonucleotides with RNA molecules of interest (ROI) encoding proteins that address specific challenges in industrial, agricultural, and biomedical applications. These polyribonucleotides are designed with microRNA (miRNA) target sequences that selectively target and regulate specific pathways. For instance, in industrial wastewater treatment, the polyribonucleotide comprises an ROI encoding a protein capable of degrading pollutants, and an miRNA target sequence designed to selectively target and degrade pollutants. Similarly, in crop yield enhancement, the polyribonucleotide comprises an ROI encoding a protein that increases plant resistance to environmental stressors, and an miRNA target sequence that selectively targets and regulates plant growth pathways.

The new claims introduce a significant shift in the application of the core technology, moving from therapeutic strategies to industrial, agricultural, and biomedical applications. The inventive concept's novelty lies in the specific design of polyribonucleotides and miRNA target sequences tailored to address distinct challenges in these fields.

Alternative embodiments of the inventive concept could include the use of different types of RNA molecules, such as siRNA or CRISPR-Cas systems, or the incorporation of additional components, like nanoparticles or lipids, to enhance delivery and efficacy. Variations of the inventive concept could also involve the application of the technology to other industries, such as cosmetics or pharmaceuticals.

The inventive concept has significant commercial potential in various industries, including industrial wastewater treatment, agriculture, biomedical research, and biofuel production. The market for RNA-based solutions is expected to grow substantially, driven by the increasing demand for sustainable and efficient solutions to pressing environmental and health challenges.