Next-Generation Flagellin-Based Vaccines and Immunotherapies

Publication ID: 24-11857614_0010_PTD
Published: October 28, 2025
Category:Future Evolutions & Paradigm Shifts

Legal Citation

pr1or.art Inc., “Next-Generation Flagellin-Based Vaccines and Immunotherapies,” Published Technical Disclosure No. 24-11857614_0010_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857614_0010_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,614.

Summary of the Inventive Concept

The present inventive concept discloses a new generation of flagellin-based vaccines and immunotherapies that leverage advanced biotechnology tools, machine learning algorithms, and nanoparticle-based adjuvants to provide enhanced immune responses, personalized treatments, and targeted therapies against bacterial infections.

Background and Problem Solved

The original patent disclosed flagellin polypeptides from Enterococcus gallinarum for treating diseases, but it had limitations in terms of immune response stimulation, specificity, and adaptability to different bacterial strains. The present inventive concept addresses these limitations by introducing recombinant flagellin polypeptides with modified domains, nanoparticle-based adjuvants, and machine learning algorithms for personalized immunotherapy.

Detailed Description of the Inventive Concept

The new claims encompass a range of innovative approaches, including the use of recombinant flagellin polypeptides with engineered D0 domains for enhanced TLR5 recognition, personalized immunotherapy systems that utilize genomic information and machine learning algorithms to predict optimal flagellin polypeptide sequences, and modular platforms for developing flagellin-based vaccines with interchangeable modules for generating recombinant polypeptides and formulating them with various adjuvants and delivery systems.

Novelty and Inventive Step

The new claims introduce several novel and non-obvious elements, including the use of nanoparticle-based adjuvants, machine learning algorithms for personalized immunotherapy, and modular platforms for developing flagellin-based vaccines. These elements provide a significant departure from the original patent and offer a new generation of flagellin-based vaccines and immunotherapies with enhanced efficacy and adaptability.

Alternative Embodiments and Variations

Other embodiments of the inventive concept could include the use of different types of adjuvants, such as liposomes or emulsions, or alternative machine learning algorithms for predicting optimal flagellin polypeptide sequences. Additionally, the modular platform could be adapted for use with different bacterial strains or diseases, providing a flexible and scalable approach to vaccine development.

Potential Commercial Applications and Market

The present inventive concept has significant commercial potential in the vaccine and immunotherapy markets, particularly in the context of bacterial infections. The ability to provide personalized and targeted therapies could revolutionize the field of immunotherapy and provide new opportunities for pharmaceutical companies, biotechnology startups, and research institutions.

CPC Classifications

SectionClassGroup
A A61 A61K39/09
A A61 A61K35/744
A A61 A61K2039/585
A A61 A61P37/04

Field of Art

Immunology, microbiology, and biotechnology, specifically focused on bacterial flagellin-based vaccine development and immunotherapeutic strategies. Requires advanced knowledge of protein engineering, immunological mechanisms, and molecular biology techniques

Person of Ordinary Skill (PHOSITA) Profile

A researcher with a PhD in microbiology or immunology, experienced in protein engineering, vaccine development, and understanding of toll-like receptor interactions. Familiar with recombinant protein techniques, machine learning applications in biotechnology, and adjuvant design

Obviousness Rationale

A person skilled in the art would recognize that the PTD represents predictable extensions of the source patent's core concept of Enterococcus flagellin polypeptides as immunological agents. The variations introduce standard biotechnological approaches like machine learning, nanoparticle adjuvants, and modular vaccine platforms that would be considered routine optimization strategies in the field of bacterial vaccine development.

Obvious Combinations & Variations

Source Patent Element
Isolated Enterococcus flagellin polypeptide with specific sequence identity to SEQ ID NO: 1
PTD Variation
Engineered flagellin polypeptides with modified D0 domains for enhanced TLR5 recognition
Obviousness Reasoning
Protein engineering to improve receptor interactions is a known technique in immunology, representing a predictable modification that a skilled practitioner would find obvious for enhancing immune response
Source Patent Element
Pharmaceutical composition for stimulating immune response
PTD Variation
Personalized immunotherapy system using machine learning to predict optimal flagellin polypeptide sequences
Obviousness Reasoning
Application of computational methods to optimize protein-based therapeutics is a standard approach in biotechnology, representing an obvious extension of existing vaccine development strategies
Source Patent Element
Enterococcus gallinarum strain-specific flagellin polypeptide
PTD Variation
Modular platform for generating recombinant flagellin polypeptides from multiple bacterial strains
Obviousness Reasoning
Creating flexible vaccine platforms with interchangeable components is a predictable design choice in biotechnology, representing a logical progression of vaccine development techniques
Source Patent Element
Pharmaceutical composition for immune stimulation
PTD Variation
Combination of flagellin polypeptides with nanoparticle adjuvants and bacteriophages
Obviousness Reasoning
Combining multiple immunological agents is a known strategy for enhancing therapeutic efficacy, representing a routine optimization approach in vaccine development
Source Patent Element
Isolated Enterococcus flagellin polypeptide
PTD Variation
Engineered polypeptides with complementary immunogenic regions for enhanced immune response
Obviousness Reasoning
Rational design of immunogenic protein regions is a standard technique in protein engineering, representing a predictable approach to improving vaccine effectiveness
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11857614 and the disclosed variations, a person having ordinary skill in the art would find the claimed innovations obvious and anticipated. The published technical disclosure demonstrates that the claimed methods represent predictable extensions of existing flagellin-based immunological strategies, utilizing standard biotechnological techniques that would be obvious to a skilled practitioner in the field of bacterial vaccine development.

Original Patent Information

Patent NumberUS 11,857,614
TitleEnterococcus gallinarum flagellin polypeptides
Assignee(s)CJ BIOSCIENCE, INC.