Multimodal Chromatography for Diverse Industrial Applications

Publication ID: 24-11857619_0002_PTD
Published: October 28, 2025
Category:New Applications & Use Cases

Legal Citation

pr1or.art Inc., “Multimodal Chromatography for Diverse Industrial Applications,” Published Technical Disclosure No. 24-11857619_0002_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857619_0002_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,619.

Summary of the Inventive Concept

The inventive concept extends the core technology of multimodal chromatography to new industries and use cases, enabling purification, separation, and detection of various biomolecules, microorganisms, and pollutants in agricultural, environmental, biomedical, and energy sectors.

Background and Problem Solved

The original patent disclosed a multimodal chromatography method for purifying HIV-1 envelope glycoprotein, demonstrating the effectiveness of this technology in biopharmaceutical applications. However, the original patent's limitations lie in its narrow focus on a specific biological molecule. This inventive concept addresses the problem of limited applicability by expanding the technology to tackle diverse industrial challenges, such as improving crop yields, treating wastewater, diagnosing diseases, producing biofuels, and removing heavy metals from soil.

Detailed Description of the Inventive Concept

The inventive concept involves the design and implementation of multimodal chromatography systems tailored to specific industrial applications. For instance, in agriculture, the system can separate and purify plant growth-promoting microorganisms to enhance crop yields. In environmental applications, the system can selectively capture and remove pollutants and contaminants from wastewater. In biomedical applications, the system can detect biomarkers in biological samples for disease diagnosis. In energy applications, the system can separate and purify biofuel-producing microorganisms. The multimodal chromatography system's versatility lies in its ability to adapt to different molecule types, sizes, and properties, making it a powerful tool for various industries.

Novelty and Inventive Step

The novelty of this inventive concept lies in its application of multimodal chromatography to entirely new industries and use cases, departing from the original patent's focus on HIV-1 envelope glycoprotein. The inventive step is the recognition of the technology's broad applicability and the development of tailored solutions for diverse industrial challenges.

Alternative Embodiments and Variations

Alternative embodiments of the inventive concept could include modifications to the multimodal resin, column design, and operating conditions to optimize performance for specific industrial applications. Variations could also involve integrating the multimodal chromatography system with other technologies, such as sensors, microfluidics, or machine learning algorithms, to enhance its capabilities and versatility.

Potential Commercial Applications and Market

The inventive concept has significant commercial potential across various industries, including agriculture, environmental remediation, biomedical diagnostics, and energy production. The target market includes companies, research institutions, and government agencies seeking innovative solutions to address pressing industrial challenges and improve their products, processes, and services.

CPC Classifications

SectionClassGroup
A A61 A61K39/21
C C07 C07K1/18
C C07 C07K14/005
C C07 C07K14/162
C C12 C12N7/02
A A61 A61K2039/53
C C12 C12N2740/16051
C C12 C12N2740/16111
C C12 C12N2740/16122
C C12 C12N2740/16134

Field of Art

Biotechnology and Chromatographic Separation Technologies, involving protein purification, biomolecule isolation, and multimodal chromatography techniques. Requires advanced degree (PhD/MS) in biochemical engineering, biotechnology, or related field with expertise in protein separation, chromatographic methods, and industrial bioprocessing

Person of Ordinary Skill (PHOSITA) Profile

Skilled practitioner with comprehensive understanding of chromatographic principles, ability to adapt separation techniques across different molecular systems, knowledge of resin chemistry, and experience in translating laboratory techniques to industrial applications

Obviousness Rationale

A PHOSITA would recognize the fundamental versatility of multimodal chromatography as a separation technique that can be readily adapted across diverse molecular systems. The core principles of selective binding, differential elution, and resin chemistry demonstrated in the HIV-1 protein purification patent inherently suggest broad applicability to other biomolecular and environmental separation challenges. The PTD's variations represent predictable extensions of known chromatographic principles by applying established separation methodologies to new technical domains.

Obvious Combinations & Variations

Source Patent Element
Multimodal resin-based protein capture technique using selective binding and elution
PTD Variation
Applying multimodal chromatography to separate agricultural microorganisms
Obviousness Reasoning
Known technique applied to a predictable alternative domain with expected similar separation mechanics
Source Patent Element
Flow-through mode of chromatographic separation used for protein purification
PTD Variation
Utilizing flow-through mode for pollutant removal from wastewater
Obviousness Reasoning
Transferring established separation principle to environmental remediation represents a straightforward technical adaptation
Source Patent Element
Selective protein capture using multimodal chromatography
PTD Variation
Detecting biomarkers through chromatographic sensor design
Obviousness Reasoning
Predictable extension of molecular capture technology into diagnostic sensing applications
Source Patent Element
Protein purification using multimodal chromatographic techniques
PTD Variation
Separating and purifying biofuel-producing microorganisms
Obviousness Reasoning
Applying known separation methodology to alternative biological system represents an obvious technical variation
35 U.S.C. § 103 Summary: Based on US Patent 11857619's teachings of multimodal chromatography for protein purification, a Person Having Ordinary Skill In The Art would find the variations disclosed herein obvious and anticipated, as they represent predictable applications of established separation techniques across diverse technical domains without requiring inventive complexity beyond routine engineering skill.

Original Patent Information

Patent NumberUS 11,857,619
TitleMultimodal chromatography method for the purification of HIV-1 envelope glycoprotein
Assignee(s)Janssen Vaccines & Prevention B.V.