Intelligent Multimodal Chromatography System for HIV-1 Envelope Glycoprotein Purification

Publication ID: 24-11857619_0003_PTD

Published: October 28, 2025

Category:Synergistic Combinations

Legal Citation

pr1or.art Inc., “Intelligent Multimodal Chromatography System for HIV-1 Envelope Glycoprotein Purification,” Published Technical Disclosure No. 24-11857619_0003_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857619_0003_PTD

This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,619.

Summary of the Inventive Concept

A novel system integrating multimodal chromatography with AI, IoT, blockchain, and other technologies to optimize and monitor the purification of HIV-1 envelope glycoprotein, enhancing efficiency, quality, and reliability.

Background and Problem Solved

The original patent disclosed a multimodal chromatography method for purifying HIV-1 envelope glycoprotein, but it lacked real-time monitoring, optimization, and security features. This inventive concept addresses these limitations by combining the original method with advanced technologies to create a more powerful and reliable system.

Detailed Description of the Inventive Concept

The system consists of a multimodal chromatography module, an AI-powered quality control module for real-time monitoring and optimization, an IoT-enabled sensor for monitoring process conditions, a blockchain-enabled tracking system for ensuring the authenticity and integrity of the resin, and a cloud-based data analytics platform for real-time data analysis and process optimization. The AI module uses machine learning algorithms to analyze data from prior purification runs and predict optimal conditions for subsequent runs. The system can be integrated with other purification technologies, such as nanofiltration or crystallization, using a modular, plug-and-play architecture.

Novelty and Inventive Step

The integration of multimodal chromatography with AI, IoT, blockchain, and other technologies provides a new, non-obvious system for purifying HIV-1 envelope glycoprotein, overcoming the limitations of the original patent. The use of machine learning algorithms, IoT-enabled sensors, and blockchain-enabled tracking systems creates a synergistic combination that enhances the efficiency, quality, and reliability of the purification process.

Alternative Embodiments and Variations

Alternative embodiments may include using different AI algorithms, integrating with other purification technologies, or incorporating additional sensors and tracking systems. Variations may include adapting the system for purifying other proteins or using different types of multimodal chromatography resins.

Potential Commercial Applications and Market

The Intelligent Multimodal Chromatography System has significant commercial potential in the biotechnology and pharmaceutical industries, particularly in the development and manufacturing of HIV vaccines and therapies. The system's ability to enhance efficiency, quality, and reliability makes it an attractive solution for companies seeking to improve their purification processes.

CPC Classifications

Section	Class	Group
A	A61	A61K39/21
C	C07	C07K1/18
C	C07	C07K14/005
C	C07	C07K14/162
C	C12	C12N7/02
A	A61	A61K2039/53
C	C12	C12N2740/16051
C	C12	C12N2740/16111
C	C12	C12N2740/16122
C	C12	C12N2740/16134

Section 103 Obviousness Analysis (PHOSITA)

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Field of Art

Biotechnology and protein purification, specifically focused on HIV envelope glycoprotein isolation techniques, requiring expertise in chromatography, protein engineering, and advanced separation technologies

Person of Ordinary Skill (PHOSITA) Profile

A PhD-level biochemical engineer or molecular biologist with expertise in protein purification methods, multimodal chromatography techniques, and advanced analytical instrumentation, familiar with emerging technologies in biotechnology process optimization

Obviousness Rationale

A PHOSITA would recognize that integrating AI, IoT, and blockchain technologies with established multimodal chromatography techniques represents a predictable application of known optimization strategies. The core purification method remains fundamentally consistent with the source patent's teachings, with technological enhancements that would be considered routine improvements in biotechnology process engineering. The proposed system extends existing chromatography principles through standard technological integration approaches.

Obvious Combinations & Variations

Source Patent Element

Multimodal chromatography method for HIV-1 gp140 protein purification

PTD Variation

AI-powered quality control module for real-time monitoring and optimization of purification conditions

Obviousness Reasoning

Implementing machine learning for process optimization is a known technique in biotechnology, representing a predictable application of computational methods to existing separation technologies

Source Patent Element

Capturing HIV-1 gp140 protein using multimodal resin

PTD Variation

IoT-enabled sensor system for monitoring process conditions and tracking resin performance

Obviousness Reasoning

Integrating sensor technologies for real-time monitoring is a standard approach in biotechnological process control, representing an obvious extension of existing quality assurance methods

Source Patent Element

Purification process for HIV-1 envelope glycoprotein

PTD Variation

Blockchain-enabled tracking system for ensuring resin authenticity and process integrity

Obviousness Reasoning

Implementing blockchain for traceability is a predictable technological enhancement in scientific and manufacturing processes, representing a logical application of emerging authentication technologies

Source Patent Element

Multimodal chromatography for protein isolation

PTD Variation

Modular, plug-and-play architecture for integrating multiple purification technologies

Obviousness Reasoning

Creating modular systems that combine complementary purification technologies is a standard design approach in biotechnology process engineering, representing an obvious optimization strategy

Source Patent Element

HIV-1 gp140 protein purification method

PTD Variation

Cloud-based data analytics platform for analyzing and optimizing purification processes

Obviousness Reasoning

Utilizing cloud computing for data analysis and process optimization is a routine technological enhancement in scientific and engineering domains, representing a predictable application of computational resources

35 U.S.C. § 103 Summary: Pursuant to 35 U.S.C. § 103, the variations disclosed in this Published Technical Disclosure would have been obvious to a Person Having Ordinary Skill In The Art at the time of invention, as the proposed technological enhancements represent predictable combinations of known techniques in multimodal chromatography and process optimization, building directly upon the foundational teachings of US Patent 11857619 through standard technological integration strategies.

Original Patent Information

Patent Number	US 11,857,619
Title	Multimodal chromatography method for the purification of HIV-1 envelope glycoprotein
Assignee(s)	Janssen Vaccines & Prevention B.V.