Multimodal Chromatography-based Therapeutic and Diagnostic Platform

Publication ID: 24-11857619_0007_PTD
Published: October 28, 2025
Category:New Applications & Use Cases

Legal Citation

pr1or.art Inc., “Multimodal Chromatography-based Therapeutic and Diagnostic Platform,” Published Technical Disclosure No. 24-11857619_0007_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857619_0007_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,619.

Summary of the Inventive Concept

This inventive concept leverages the core technology of multimodal chromatography for the purification of proteins, expanding its application to new industries, including neurodegenerative disease treatment, cancer therapy, and disease diagnosis.

Background and Problem Solved

The original patent disclosed a multimodal chromatography method for purifying HIV-1 envelope glycoprotein. However, the technology's potential extends beyond this specific application. The inventive concept addresses the need for efficient and effective protein purification methods in various fields, where the original patent's limitations are overcome by adapting the technology to new use cases.

Detailed Description of the Inventive Concept

The new inventive concept comprises a multimodal chromatography-based platform for purifying proteins, which can be used in various therapeutic and diagnostic applications. The platform consists of a multimodal resin, a buffer system, and a pump for flowing the buffer through the column. The protein of interest is captured on the multimodal resin and eluted in a buffer suitable for administration to a patient or for use in diagnostic assays. The system can be used for treating neurodegenerative diseases, cancer, and other diseases, as well as for diagnosing diseases using multimodal chromatography-purified proteins.

Novelty and Inventive Step

The new claims introduce novel applications and use cases for the multimodal chromatography technology, which were not originally considered in the context of HIV-1 envelope glycoprotein purification. The inventive step lies in the adaptation of the core technology to new industries and fields, providing a significant expansion of its potential impact.

Alternative Embodiments and Variations

Alternative embodiments of the inventive concept may include different types of multimodal resins, buffer systems, and pump configurations. Variations may also include the use of different protein targets, disease indications, or diagnostic assays. Additionally, the platform could be integrated with other technologies, such as microfluidics or biosensors, to enhance its functionality and versatility.

Potential Commercial Applications and Market

The multimodal chromatography-based therapeutic and diagnostic platform has significant commercial potential in various industries, including pharmaceuticals, biotechnology, and diagnostics. The platform's adaptability to different protein targets and disease indications makes it an attractive solution for companies seeking to develop new therapies and diagnostic tools. The target market includes pharmaceutical companies, biotech startups, and diagnostic companies seeking to develop innovative solutions for unmet medical needs.

CPC Classifications

SectionClassGroup
A A61 A61K39/21
C C07 C07K1/18
C C07 C07K14/005
C C07 C07K14/162
C C12 C12N7/02
A A61 A61K2039/53
C C12 C12N2740/16051
C C12 C12N2740/16111
C C12 C12N2740/16122
C C12 C12N2740/16134

Field of Art

Protein purification, biotechnology, and biomedical engineering, with expertise in chromatographic separation techniques, protein isolation, and therapeutic protein processing

Person of Ordinary Skill (PHOSITA) Profile

A skilled practitioner with advanced degree in biochemical engineering or molecular biology, experienced in protein purification methods, multimodal chromatography techniques, and biotechnological process development

Obviousness Rationale

A person of ordinary skill would recognize the fundamental versatility of multimodal chromatography as a generalizable protein purification technique that can be readily adapted across different protein targets and therapeutic applications. The core technological principles demonstrated in the HIV-1 gp140 purification method are directly transferable to other protein isolation scenarios, with predictable outcomes and minimal inventive complexity. The PTD's expansion of the multimodal chromatography platform to neurodegenerative diseases, cancer therapy, and diagnostic applications represents a straightforward technical extension of the source patent's core methodology.

Obvious Combinations & Variations

Source Patent Element
Multimodal resin-based protein capture and elution method for HIV-1 gp140
PTD Variation
Applying identical multimodal chromatography technique to purify therapeutic proteins for neurodegenerative disease treatment
Obviousness Reasoning
Known technique applied to a predictable alternative protein target with expected similar purification outcomes
Source Patent Element
Flow-through mode of multimodal chromatography resin
PTD Variation
Extending flow-through mode to protein purification for cancer diagnostic and therapeutic applications
Obviousness Reasoning
Established chromatographic technique can be trivially adapted to different protein separation contexts with predictable results
Source Patent Element
Protein capture and elution using specific buffer systems
PTD Variation
Modifying buffer composition for different protein targets while maintaining core multimodal chromatography principles
Obviousness Reasoning
Buffer optimization is a routine design choice for skilled practitioners, representing an obvious variation within known technical parameters
Source Patent Element
Protein purification method involving multimodal resin interactions
PTD Variation
Integrating multimodal chromatography with complementary technologies like microfluidics or biosensors
Obviousness Reasoning
Combining known techniques in a predictable manner to enhance technological functionality is an obvious engineering approach
Source Patent Element
Specific protein purification workflow for HIV envelope glycoprotein
PTD Variation
Creating a generalized protein purification kit applicable across multiple disease contexts
Obviousness Reasoning
Developing a standardized purification platform based on established chromatographic principles represents a logical technical progression
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11857619, a person having ordinary skill in the art would find the protein purification variations disclosed herein to be obvious extensions of the fundamental multimodal chromatography methodology. The proposed technical adaptations represent predictable applications of known protein isolation techniques, demonstrating a lack of non-obvious inventive step sufficient to distinguish from the prior art.

Original Patent Information

Patent NumberUS 11,857,619
TitleMultimodal chromatography method for the purification of HIV-1 envelope glycoprotein
Assignee(s)Janssen Vaccines & Prevention B.V.