Phosphorothioate-Conjugated miRNAs for Diverse Industrial Applications

Publication ID: 24-11857633_0007_PTD
Published: October 28, 2025
Category:New Applications & Use Cases

Legal Citation

pr1or.art Inc., “Phosphorothioate-Conjugated miRNAs for Diverse Industrial Applications,” Published Technical Disclosure No. 24-11857633_0007_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857633_0007_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,633.

Summary of the Inventive Concept

Expanding the core technology of phosphorothioate-conjugated miRNAs to address unmet needs in environmental monitoring, agriculture, diagnostics, personalized medicine, and skin health

Background and Problem Solved

The original patent focused on treating diseases, particularly multiple myeloma and IL-6 signaling. However, the technology's potential extends beyond disease treatment. The new inventive concept addresses the limitations of the original patent by exploring novel applications and use cases, unlocking the technology's broader industrial potential

Detailed Description of the Inventive Concept

The new inventive concept leverages phosphorothioate-conjugated miRNAs in various industries. For instance, in environmental monitoring, miRNA sensors can detect specific pollutants. In agriculture, phosphorothioate-conjugated miRNAs can regulate genes involved in plant growth and development, improving crop yields. In diagnostics, miRNA probes can bind to biomarkers of neurological disorders. In personalized medicine, computational platforms can identify optimal miRNA combinations for treating specific diseases. Finally, in skin health, topical administration of phosphorothioate-conjugated miRNAs can regulate genes involved in skin inflammation

Novelty and Inventive Step

The new claims introduce novel applications and use cases that were not originally considered, expanding the technology's scope beyond disease treatment. The inventive step lies in the recognition of the technology's potential in diverse industrial settings and the development of new, non-obvious applications

Alternative Embodiments and Variations

Other potential embodiments include using phosphorothioate-conjugated miRNAs in bioremediation, biotechnology, or cosmetics. Variations may involve different miRNA sequences, conjugation methods, or delivery mechanisms

Potential Commercial Applications and Market

The inventive concept has significant commercial potential in various industries, including environmental monitoring, agriculture, diagnostics, personalized medicine, and skin health. The market size for these industries is substantial, and the technology's adoption could lead to significant revenue growth and societal impact

CPC Classifications

SectionClassGroup
A A61 A61K47/549
A A61 A61K47/548
A A61 A61K48/00
C C12 C12N15/113
C C12 C12N2310/141
C C12 C12N2320/30

Field of Art

Molecular biology, biotechnology, and nucleic acid therapeutics, with expertise in microRNA conjugation, delivery mechanisms, and therapeutic applications across multiple domains

Person of Ordinary Skill (PHOSITA) Profile

A researcher with advanced degrees in molecular biology or biotechnology, experienced in nucleic acid design, conjugation techniques, and understanding of miRNA functionality, capable of interdisciplinary problem-solving and technology translation

Obviousness Rationale

A PHOSITA would recognize that phosphorothioate-conjugated miRNAs represent a versatile molecular platform with inherent potential for broad application beyond initial disease treatment. The source patent's fundamental technology of miRNA conjugation provides a clear technological foundation that can be systematically extended to diverse industrial contexts. The disclosed variations represent predictable extrapolations of known molecular engineering principles applied to specific domain challenges.

Obvious Combinations & Variations

Source Patent Element
Phosphorothioate-conjugated miRNA delivery method for treating multiple myeloma
PTD Variation
Environmental monitoring using miRNA sensors to detect pollutants
Obviousness Reasoning
A PHOSITA would recognize that the core conjugation technology enabling cellular penetration and molecular specificity is directly transferable to sensing applications, representing a known technique for expanding technological utility
Source Patent Element
miRNA sequences targeting specific cellular signaling pathways
PTD Variation
Agricultural applications regulating plant growth genes
Obviousness Reasoning
The fundamental mechanism of gene regulation through miRNA is well-established, making cross-domain application a predictable extension of existing molecular biology principles
Source Patent Element
Nucleic acid conjugation for targeted cellular delivery
PTD Variation
Personalized medicine computational platforms identifying optimal miRNA combinations
Obviousness Reasoning
Computational screening of molecular interactions is a standard technique, and the source patent's molecular design provides a clear technological substrate for systematic exploration
Source Patent Element
Phosphorothioate chemical modification enabling enhanced molecular stability
PTD Variation
Topical skin health applications targeting inflammatory genes
Obviousness Reasoning
The chemical modification's ability to enhance molecular stability and penetration makes its application across different tissue types a predictable design choice for a skilled practitioner
Source Patent Element
miRNA sequences targeting specific cellular pathways
PTD Variation
Diagnostic biomarker detection for neurological disorders
Obviousness Reasoning
Molecular probes designed for specific pathway interactions are a known technique, and extending this to diagnostic applications represents a standard technological progression
35 U.S.C. § 103 Summary: Pursuant to 35 U.S.C. ยง 103, the variations disclosed in this publication would have been obvious to a person having ordinary skill in the art at the time of invention, given the teachings of US Patent 11857633. The fundamental phosphorothioate-conjugated miRNA technology disclosed in the source patent provides a comprehensive technological foundation that renders the claimed industrial and diagnostic applications predictable extensions of known molecular engineering principles.

Original Patent Information

Patent NumberUS 11,857,633
TitlePhosphorothioate-conjugated miRNAs and methods of using the same
Assignee(s)City of Hope