Next-Generation Phosphorothioate-conjugated miRNAs for Personalized Medicine

Publication ID: 24-11857633_0010_PTD
Published: October 28, 2025
Category:Future Evolutions & Paradigm Shifts

Legal Citation

pr1or.art Inc., “Next-Generation Phosphorothioate-conjugated miRNAs for Personalized Medicine,” Published Technical Disclosure No. 24-11857633_0010_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857633_0010_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,633.

Summary of the Inventive Concept

A novel, next-generation technology for treating multiple myeloma and other diseases using phosphorothioate-conjugated miRNAs, incorporating advanced delivery systems, personalized medicine approaches, and real-time monitoring capabilities.

Background and Problem Solved

The original patent disclosed phosphorothioate-conjugated miRNAs for treating multiple myeloma, but it had limitations in terms of delivery, efficacy, and patient specificity. The new inventive concept addresses these limitations by introducing nanoparticle-based delivery, personalized medicine approaches, and real-time monitoring capabilities, enabling more effective and targeted treatment of diseases.

Detailed Description of the Inventive Concept

The next-generation technology comprises a nucleic acid conjugate with a microRNA selected from the group consisting of let7a-3p miRNA, let7a-5p miRNA, miR17-3p miRNA, miR17-5p miRNA, and miR218-5p miRNA. The conjugate is delivered via a nanoparticle with a tunable release profile, allowing for controlled and sustained release of the microRNA. Additionally, the technology incorporates a device for monitoring treatment efficacy and a bioinformatics tool for predicting treatment outcomes, enabling personalized medicine approaches. The system also includes a composition of matter comprising a nucleic acid conjugate with a microRNA and a polymer matrix with tunable biodegradability, which is implantable and releases the conjugate in a controlled manner.

Novelty and Inventive Step

The new claims introduce several novel and non-obvious features, including nanoparticle-based delivery, personalized medicine approaches, and real-time monitoring capabilities, which are not present in the original patent. The combination of these features enables a more effective and targeted treatment of diseases, which is not obvious from the original patent.

Alternative Embodiments and Variations

Alternative embodiments of the inventive concept could include different types of nanoparticles, such as liposomes or dendrimers, or different polymer matrices with varying biodegradability profiles. Additionally, the technology could be adapted for use with different microRNAs or nucleic acid conjugates, or for treating different diseases.

Potential Commercial Applications and Market

The next-generation technology has significant commercial potential in the treatment of multiple myeloma and other diseases, particularly in the context of personalized medicine. The technology could be licensed to pharmaceutical companies or used to develop new products and services in the healthcare industry.

CPC Classifications

SectionClassGroup
A A61 A61K47/549
A A61 A61K47/548
A A61 A61K48/00
C C12 C12N15/113
C C12 C12N2310/141
C C12 C12N2320/30

Field of Art

Molecular Biology and Pharmaceutical Biotechnology, focusing on microRNA delivery systems, nucleic acid conjugation, and personalized medicine technologies with expertise in nanoparticle drug delivery and genetic therapeutic interventions

Person of Ordinary Skill (PHOSITA) Profile

A skilled practitioner with advanced degrees in molecular biology or pharmaceutical sciences, experienced in microRNA manipulation, drug delivery system design, and understanding of conjugation techniques for therapeutic nucleic acids

Obviousness Rationale

A person of ordinary skill would recognize that the PTD's disclosed variations represent predictable extensions of the source patent's foundational microRNA conjugation technology. The nanoparticle delivery, personalized medicine approaches, and monitoring systems are logical technological progressions that would be apparent to a skilled researcher seeking to improve therapeutic efficacy and patient-specific treatment strategies. The core microRNA selection remains consistent with the source patent, while the additional technological layers represent incremental yet obvious innovations in the field.

Obvious Combinations & Variations

Source Patent Element
Phosphorothioate-conjugated microRNA targeting specific disease pathways
PTD Variation
Nanoparticle-based delivery system with tunable release profile for the same microRNA set
Obviousness Reasoning
Nanoparticle drug delivery is a well-established technique in the art, and adapting the existing microRNA conjugates to controlled-release systems would be an obvious optimization for a skilled practitioner seeking improved therapeutic performance
Source Patent Element
Multiple myeloma treatment method using specific microRNA conjugates
PTD Variation
Personalized medicine approach with bioinformatics tool for predicting treatment outcomes
Obviousness Reasoning
Integrating computational predictive tools with existing therapeutic approaches is a known strategy in precision medicine, representing a predictable technological progression for improving treatment specificity
Source Patent Element
Nucleic acid conjugation technique for cellular delivery
PTD Variation
Implantable polymer matrix with tunable biodegradability for sustained microRNA release
Obviousness Reasoning
Controlled-release implantable systems are a standard approach in drug delivery, and applying this technique to the existing microRNA conjugation technology would be an obvious design choice for a skilled researcher
Source Patent Element
microRNA targeting for cellular signaling modulation
PTD Variation
Real-time monitoring device with microfluidic chip for tracking microRNA levels
Obviousness Reasoning
Development of monitoring technologies for therapeutic interventions is a predictable technological advancement, particularly in personalized medicine approaches targeting specific molecular pathways
Source Patent Element
Nucleic acid conjugation methodology
PTD Variation
High-throughput screening system for automated synthesis and testing of conjugates
Obviousness Reasoning
Automation and systematic screening are standard techniques in biotechnological research, representing an obvious method for expanding and optimizing existing therapeutic approaches
35 U.S.C. § 103 Summary: Based on the teachings of US Patent 11857633 and the comprehensive technological extensions disclosed herein, a person of ordinary skill in the art would find the claimed variations obvious and predictable. The published technical disclosure demonstrates that the proposed innovations represent straightforward technological progressions that would be apparent to a skilled practitioner, thereby rendering potential patent claims in this domain anticipated and non-patentable under standard obviousness criteria.

Original Patent Information

Patent NumberUS 11,857,633
TitlePhosphorothioate-conjugated miRNAs and methods of using the same
Assignee(s)City of Hope