Synergistic Fluorescence Enhancement Systems
Legal Citation
Summary of the Inventive Concept
This inventive concept integrates fluorescent dyes with AI, IoT, blockchain, and new materials to create powerful systems for enhanced fluorescence signals and reduced quenching in biomolecules.
Background and Problem Solved
The original patent, 'Compositions and methods for enhanced fluorescence', addressed the limitations of fluorescently labeled biomolecules in quantitation assays and cellular imaging. However, it did not explore the potential of combining these compositions with emerging technologies to further enhance fluorescence signals and reduce quenching. This inventive concept solves this problem by introducing synergistic combinations of fluorescent dyes with AI, IoT, blockchain, and new materials.
Detailed Description of the Inventive Concept
The inventive concept comprises four main systems: 1) AI-powered optimization of dye-to-protein ratios to reduce quenching; 2) IoT-enabled microscopes for real-time fluorescence imaging and analysis; 3) compositions incorporating nano-particles with metal oxide materials for enhanced fluorescence signals; and 4) microfluidic devices for controlling dye-to-protein ratios and preventing quenching. These systems leverage AI, IoT, blockchain, and new materials to create more powerful and efficient fluorescence enhancement solutions.
Novelty and Inventive Step
The new claims introduce the novel concept of integrating fluorescent dyes with AI, IoT, blockchain, and new materials to create synergistic systems for enhanced fluorescence signals and reduced quenching. This integration is non-obvious and represents a significant departure from the original patent, which focused solely on compositions and methods for enhancing fluorescence.
Alternative Embodiments and Variations
Alternative embodiments of the inventive concept could include the use of different AI algorithms, IoT-enabled devices, or blockchain-based data storage systems. Variations could also include the integration of additional technologies, such as robotics or advanced materials, to further enhance fluorescence signals and reduce quenching.
Potential Commercial Applications and Market
The inventive concept has significant commercial potential in the life sciences and biotechnology industries, where fluorescence-based assays and imaging techniques are widely used. The market for fluorescence-based solutions is expected to grow significantly in the coming years, driven by the increasing demand for advanced diagnostic and research tools.
CPC Classifications
| Section | Class | Group |
|---|---|---|
| A | A61 | A61K49/0015 |
| A | A61 | A61K47/10 |
| A | A61 | A61K47/18 |
| A | A61 | A61K49/0058 |
| G | G01 | G01N33/533 |
| G | G01 | G01N33/582 |
| G | G01 | G01N33/68 |
Section 103 Obviousness Analysis (PHOSITA)
Field of Art
Biomedical engineering and molecular biotechnology, focusing on fluorescence labeling techniques, biomolecule modification, and advanced imaging technologies. Requires expertise in protein chemistry, optical imaging, and computational analysis of molecular interactions
Person of Ordinary Skill (PHOSITA) Profile
A researcher with advanced degree in bioengineering or molecular biology, proficient in protein conjugation techniques, fluorescence microscopy, computational methods, and familiar with emerging technologies in biomolecular imaging and signal enhancement
Obviousness Rationale
A person skilled in the art would recognize that the PTD's integration of AI, IoT, and computational techniques represents a predictable extension of existing fluorescence enhancement methods. The core technical problem of reducing fluorescent quenching remains consistent with the source patent, while the proposed solutions leverage standard computational and engineering approaches known in the field. The variations represent incremental improvements using well-established technological integration strategies.
Obvious Combinations & Variations
Original Patent Information
| Patent Number | US 11,857,643 |
|---|---|
| Title | Compositions and methods for enhanced fluorescence |
| Assignee(s) | Life Technologies Corporation, Pierce Biotechnology, Inc. |