Enhanced Multi-Signal Fluorescent Probe for Early Tumor Diagnosis
Legal Citation
Summary of the Inventive Concept
An improved multi-signal fluorescent probe for early tumor diagnosis, featuring a modified structural formula with a hydrophilic linker, solvent-free microwave-assisted synthesis, and optional fluorescent quencher and biocompatible nanoparticle encapsulation.
Background and Problem Solved
The original multi-signal fluorescent probe, disclosed in Chinese Patent Application No. 202210441449.6, had limitations in terms of solubility, bioavailability, and reaction efficiency. The new inventive concept addresses these limitations by introducing a hydrophilic linker, microwave-assisted reaction, and optional fluorescent quencher and biocompatible nanoparticle encapsulation, resulting in an enhanced probe with improved performance and efficiency.
Detailed Description of the Inventive Concept
The enhanced multi-signal fluorescent probe features a modified structural formula incorporating a hydrophilic linker, which improves its solubility and bioavailability. The probe can be synthesized using a solvent-free microwave-assisted reaction, reducing reaction time and improving yield. Additionally, the reaction can be performed at a controlled temperature of 80-90°C to optimize reaction efficiency. The probe can further be modified by incorporating a fluorescent quencher to minimize background fluorescence and improve signal-to-noise ratio. Finally, the probe can be encapsulated in a biocompatible nanoparticle to enhance its stability and targeting efficiency.
Novelty and Inventive Step
The new inventive concept introduces a hydrophilic linker, microwave-assisted synthesis, and optional fluorescent quencher and biocompatible nanoparticle encapsulation, which are not present in the original patent. These features provide a significant improvement in the probe's performance and efficiency, making the new concept novel and non-obvious.
Alternative Embodiments and Variations
Alternative embodiments of the enhanced probe could include different types of hydrophilic linkers, varying microwave-assisted reaction conditions, and different fluorescent quenchers or biocompatible nanoparticles. These variations could be used to optimize the probe's performance for specific applications or target tissues.
Potential Commercial Applications and Market
The enhanced multi-signal fluorescent probe has significant commercial potential in the field of early tumor diagnosis, particularly in the development of point-of-care diagnostic devices and personalized medicine. The target market includes research institutions, hospitals, and biotechnology companies focused on cancer diagnosis and treatment.
CPC Classifications
| Section | Class | Group |
|---|---|---|
| A | A61 | A61K49/003 |
Section 103 Obviousness Analysis (PHOSITA)
Field of Art
Analytical chemistry, specifically fluorescent probes for medical diagnostics, with expertise in organic synthesis, spectroscopic techniques, and biomedical imaging
Person of Ordinary Skill (PHOSITA) Profile
A researcher with a PhD in chemistry or biomedical engineering, experienced in organic synthesis, fluorescence spectroscopy, and medical diagnostic probe design, familiar with nanoparticle encapsulation and reaction optimization techniques
Obviousness Rationale
A person having ordinary skill in the art would recognize that the proposed modifications to the multi-signal fluorescent probe represent predictable variations that would be obvious to implement. The core structural elements of the original probe remain consistent, with incremental improvements in synthesis methodology and probe performance that are well within the standard problem-solving approaches in analytical chemistry and diagnostic probe development.
Obvious Combinations & Variations
Original Patent Information
| Patent Number | US 11,857,644 |
|---|---|
| Title | Multi-signal fluorescent probe for early diagnosis of tumors, and preparation and use thereof |
| Assignee(s) | Hunan Normal University, Hunan Cancer Hospital |