Multi-signal Fluorescent Probe for Diverse Applications

Publication ID: 24-11857644_0002_PTD
Published: October 28, 2025
Category:New Applications & Use Cases

Legal Citation

pr1or.art Inc., “Multi-signal Fluorescent Probe for Diverse Applications,” Published Technical Disclosure No. 24-11857644_0002_PTD, Published October 28, 2025, available at https://archive.pr1or.art/24-11857644_0002_PTD
This technical disclosure describes improvements that would be readily apparent to a Person Having Ordinary Skill In The Art (PHOSITA) when considered in combination with the foundational architecture disclosed in U.S. Patent No. 11,857,644.

Summary of the Inventive Concept

This inventive concept extends the core technology of the original multi-signal fluorescent probe to various industries, enabling early detection of pollutants, diseases, spoilage, and biofilm formation, thereby improving environmental sustainability, public health, and food safety.

Background and Problem Solved

The original patent disclosed a multi-signal fluorescent probe for early diagnosis of tumors. However, its application was limited to the medical field. This inventive concept addresses the need for a versatile, adaptable probe that can be applied to diverse industries, solving the problem of limited scope and applicability.

Detailed Description of the Inventive Concept

The inventive concept comprises a multi-signal fluorescent probe adapted for various applications, including environmental pollution monitoring, crop disease diagnosis, food spoilage detection, air quality monitoring, and biofilm formation detection. The probe emits distinct signals indicative of specific contaminants or pathogens, enabling early detection and targeted interventions. The probe's versatility stems from its ability to be tailored to different industries, with modifications to its structural formula, preparation methods, and signal emission profiles.

Novelty and Inventive Step

The novelty of this inventive concept lies in its extension of the original probe's technology to entirely new industries, solving previously unaddressed problems. The inventive step involves adapting the probe's design and functionality to accommodate diverse applications, thereby creating a versatile, high-impact technology.

Alternative Embodiments and Variations

Alternative embodiments of the inventive concept may include modifications to the probe's structural formula, such as using different fluorophores or conjugating molecules. Variations may also involve changes to the preparation methods, signal emission profiles, or detection systems to optimize the probe's performance for specific applications.

Potential Commercial Applications and Market

The inventive concept has significant commercial potential across various industries, including environmental monitoring, agriculture, food safety, air quality management, and biomedical research. The target market includes government agencies, research institutions, and private companies operating in these sectors, with potential applications in early detection, prevention, and mitigation of diverse hazards.

CPC Classifications

SectionClassGroup
A A61 A61K49/003

Field of Art

Analytical chemistry, fluorescence-based diagnostic and detection technologies, with expertise in molecular probe design, signal detection, and multi-functional sensing systems

Person of Ordinary Skill (PHOSITA) Profile

A researcher or engineer with advanced degree in chemistry, biochemistry, or related field, experienced in fluorescent probe synthesis, signal transduction mechanisms, and interdisciplinary application of molecular sensing technologies

Obviousness Rationale

A PHOSITA would recognize that the core fluorescent probe technology disclosed in the source patent provides a fundamental platform for adaptation across multiple detection domains. The multi-signal emission characteristics inherently suggest versatile applicability beyond tumor diagnosis. The structural flexibility of the probe and its signal generation mechanism naturally lend themselves to environmental, agricultural, and industrial monitoring applications.

Obvious Combinations & Variations

Source Patent Element
Multi-signal fluorescent probe with distinct signal emission capabilities
PTD Variation
Extending probe functionality to environmental pollution detection with heavy metal and organic pollutant signals
Obviousness Reasoning
Known technique of adapting fluorescent probes for different detection scenarios, with predictable signal transduction principles applicable across diverse detection targets
Source Patent Element
Structural formula allowing molecular modifications
PTD Variation
Tailoring probe to detect crop diseases through fungal and bacterial infection signals
Obviousness Reasoning
Predictable result of modifying fluorophore conjugation to enable specific pathogen detection, utilizing standard molecular engineering approaches
Source Patent Element
Signal generation mechanism for tumor diagnosis
PTD Variation
Repurposing probe for food spoilage detection with bacterial and fungal presence indicators
Obviousness Reasoning
Finite set of known detection strategies that a PHOSITA would recognize as straightforward extension of existing molecular probe technology
Source Patent Element
Preparation methods involving chemical modification of base molecular structure
PTD Variation
Developing air quality monitoring probe detecting particulate matter and volatile organic compounds
Obviousness Reasoning
Known technique of adapting probe synthesis to introduce selective binding and signal generation for different molecular targets
Source Patent Element
Multi-signal fluorescence detection principle
PTD Variation
Creating biofilm formation detection system with distinct bacterial and fungal signals
Obviousness Reasoning
Predictable application of existing signal transduction technology to expand probe's diagnostic capabilities across different biological contexts
35 U.S.C. § 103 Summary: Pursuant to 35 U.S.C. ยง 103, the variations disclosed herein would have been obvious to a person having ordinary skill in the art at the time of invention, based on the foundational teachings of US Patent 11857644. The multi-signal fluorescent probe technology, when viewed through the lens of its inherent adaptability and signal generation principles, renders obvious the extension of its diagnostic capabilities across environmental, agricultural, and industrial domains.

Original Patent Information

Patent NumberUS 11,857,644
TitleMulti-signal fluorescent probe for early diagnosis of tumors, and preparation and use thereof
Assignee(s)Hunan Normal University, Hunan Cancer Hospital